diff -r 0b0e72b5d551 -r 0e99b876966a mercurial/manifest.py
--- a/mercurial/manifest.py	Tue Jan 07 13:47:29 2020 -0500
+++ b/mercurial/manifest.py	Thu Apr 02 15:52:10 2020 -0400
@@ -1027,7 +1027,12 @@
                 self._dirs[dir] = treemanifest(self._subpath(dir))
             self._dirs[dir].__setitem__(subpath, n)
         else:
-            self._files[f] = n[:21]  # to match manifestdict's behavior
+            # manifest nodes are either 20 bytes or 32 bytes,
+            # depending on the hash in use. An extra byte is
+            # occasionally used by hg, but won't ever be
+            # persisted. Trim to 21 or 33 bytes as appropriate.
+            trim = 21 if len(n) < 25 else 33
+            self._files[f] = n[:trim]  # to match manifestdict's behavior
         self._dirty = True
 
     def _load(self):